Journal: Scientific Reports

Article Title: Propolis potentiates the effect of cranberry ( Vaccinium macrocarpon ) against the virulence of uropathogenic Escherichia coli

doi: 10.1038/s41598-018-29082-6

Figure Lengend Snippet: Microarray results. Proportion of genes up-regulated and down-regulated by cranberry (190 μg/mL) and propolis (102.4 μg/mL) alone and combined. Cranberry up-regulates 1,245 genes and down-regulates 2,190 genes. Propolis up-regulates 94 genes and down- regulates 1,384 genes. Cranberry + propolis up-regulate 2,950 genes and down-regulate 2,150 genes.

Article Snippet: The one-color microarray-based prokaryote analysis protocol (FairplayIII Microarray labelling, Agilent) was used according to the manufacturer’s instructions to synthetize and label cDNA.

Techniques: Microarray

Journal: BioMed Research International

Article Title: Update on the Pathogenic Implications and Clinical Potential of microRNAs in Cardiac Disease

doi: 10.1155/2015/105620

Figure Lengend Snippet: The literature review summary of miRNAs found to be deregulated in cardiovascular diseases. A number of studies have used high throughput miRNA profiling technologies to compare miRNA expression levels between control and affected tissues from cardiac disease inducing mice models or postmortem samples from failing human myocardium. The heading of the table identifies the specific study, followed by the species and diseases modeled, and the experimental design as well as the microarray technologies used to analyze the data. In bold, in rows below, the most deregulated miRNAs according to the different studies are displayed. The following miRNAs tabulated that are not in bold represent other cardiac specific miRNAs found to be deregulated in the five studies referenced but not directly mentioned in our review. Arrows illustrate the fold change of upregulation or downregulation of listed miRNAs as compared to control samples. miRNAs with the same seed region were combined into families. The family was labeled as regulated when at least 3 members of the family displayed a change in expression profile. Each family includes the following: let-7 family = let-7a, let-b, let-c, let-d, let-e, let-f, let-g, let-h, let-I, and let-j; miR-15 family = miR-15a, miR-15b, miR-16, miR-195, miR-424, and miR-497; miR-17 family = miR-17-5p, miR-20a, miR-20b, miR-93, miR-106a, and miR-106b; miR-29 family = miR-29a, miR-29b, and miR-29c; miR-30 family = miR-30a, miR-30b, miR-30c, miR-30d, and miR-30e. LAC = left coronary artery constriction; HF = heart failure; ICM = ischemic cardiomyopathy; DCM = dilated cardiomyopathy; d = days. ↑↑↑ ≥ 7.5; ↑↑ ≥ 2.5; ↑ ≥ 1 fold increase; —: not available; ↓ ≥ −1; ↓↓ ≥ −2.5; ↓↓↓ ≥ −7.5 fold decrease.

Article Snippet: Microarray platform , miRMAX microarray , , , Bead-based flow cytometric miRNA , , , Ncode microarray , , Illumina microarray , mirVana microarray , , .

Techniques: High Throughput Screening Assay, Expressing, Control, Microarray, Labeling

Journal: PLoS ONE

Article Title: Identification of Novel Low-Dose Bisphenol A Targets in Human Foreskin Fibroblast Cells Derived from Hypospadias Patients

doi: 10.1371/journal.pone.0036711

Figure Lengend Snippet: ( A ) “Endocrine System Disorders, Gastrointestinal Disease, Genetic Disorder” network and ( B ) “Cell Death, Cellular Growth and Proliferation, Cancer” network. The images were created using the IPA platform by overlaying the differentially expressed genes in response to BPA detected by Agilent microarray analysis onto a global molecular network from the Ingenuity knowledgebase. Red indicates upregulated genes, green indicates downregulated genes, and white indicates genes that were not annotated in this array but that form part of this network. The bottom numbers indicate the fold changes induced by BPA, and the top numbers are the P -values between the DMSO control group and the BPA treated group. Direct relationships are exhibited with solid arrows and indirect relationships with dashed arrows.

Article Snippet: RNA was amplified into cRNA and labeled according to the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technologies).

Techniques: Microarray